sspE [2019-06-25 09:32:15]

small acid-soluble spore protein (major gamma-type SASP)

locus: BSU08660

pI: 8.94

mw: 9.13 kDa

protein length: 84 aa

gene length: 255 bp

function: protection of spore DNA

product: small acid-soluble spore protein (major gamma-type SASP)

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.3|Small acid-soluble spore proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 937,900 938,154

The protein

Protein family

• gamma-type SASP family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|5964B6E817260DA7937796DDFA753A665A04D650|fabL]-[gene|25FC6EEC336387285724F75E304B76A2A3E1C056|sspE]
  description: [Pubmed|10463184]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10463184], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT]: repression, in [regulon|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|SpoVT regulon]
  • [protein|BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|YfhP]: repression, in [regulon|BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|YfhP regulon]

  regulation

  • repressed by casamino acids [Pubmed|12107147]

• genes: [gene|B62083CF18FC4C796D95B960CF62FA963FC26CDE|mutY]-[gene|5964B6E817260DA7937796DDFA753A665A04D650|fabL]-[gene|25FC6EEC336387285724F75E304B76A2A3E1C056|sspE]
  description: [Pubmed|10463184]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10463184], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|1900507], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|YfhP]: repression, in [regulon|BB4007ADF92649730C9F9A26FD082ABC8CFA1E48|YfhP regulon]

  regulation

  • expression is constitutive throughout growth [Pubmed|20971907]

additional information

• the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]

Biological materials

Mutant

• MGNA-C365 (sspE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2363 NBRP B. subtilis, Japan]
• 1S112 (no resistance), [Pubmed|3125155], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S112&Search=1S112 BGSC]
• 1S113 (no resistance), [Pubmed|3125155], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S113&Search=1S113 BGSC]
• BKE08660 ([gene|25FC6EEC336387285724F75E304B76A2A3E1C056|sspE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTATCACCTCCACGG, downstream forward: _UP4_TAATCACTGAAACAGAAAAA
• BKK08660 ([gene|25FC6EEC336387285724F75E304B76A2A3E1C056|sspE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTATCACCTCCACGG, downstream forward: _UP4_TAATCACTGAAACAGAAAAA

References

• 3106326,1900507,2456528,2497051,11092849,12107147,10463184,23314362,30782632